Journal: Frontiers in Microbiology
Article Title: Genomic analysis of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) causing infections in children—a Spanish multicenter study
doi: 10.3389/fmicb.2025.1534840
Figure Lengend Snippet: Minimum spanning tree of 98 CA-MRSA isolates causing children infections in Spain based on a cgMLST scheme of 1,861 target genes (SeqSphere+ Ridom, GmbH, Münster, Germany). A threshold of ≤ 5 allelic differences has been applied to highlight in grey shadow genetically related isolates. Isolate names (“C2” refers to study COSACO 2 CA-MRSA infections; “Sau” refers to Staphylococcus aureus ; followed by internal numbers assigned), STs and spa -types are indicated for each isolate. Each circle represents an isolate unless more than one isolate having no allele differences are grouped, in this case two or more different names are displayed. Colors indicate ST and have been used only with STs found more than once (white color indicates STs represented with one unique isolate). Groups observed are (grey shadow): C2Sau080 and C2Sau085 (4 allele differences), t005, ST22; C2Sau084, C2Sau091, C2Sau092 (3–5 allele differences), t008, ST8; C2Sau065 and C2Sau066 (2 allele differences), t008, ST8; C2Sau057 and C2Sau075 (0 allele differences), t002, ST5.
Article Snippet: Genome assemblies were uploaded to Ridom SeqSphere+ v9.0 commercial software (Münster, Germany) ( ) for a gene-by-gene comparison using a cgMLST scheme of 1,861 targets and to obtain ST-MLST ( ; ) and spa -types.
Techniques: